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Article

A region of the broad-host-range plasmid RK2 causes stable in planta inheritance of plasmids in Rhizobium meliloti cells isolated from alfalfa root nodules.

Journal of bacteriology - 1 Nov 1992

Weinstein M, Roberts R C, Helinski D R

Abstract excerpt

We demonstrate for the first time that the broad-host-range stabilization loci from plasmid RK2 cause total retention of plasmids in cells of Rhizobium meliloti during symbiosis with alfalfa. Two derivatives of plasmid RK2, pRK290 and a 7.3-kb mini-RK2 plasmid, were stabilized in R. meliloti cells isolated from root nodules by the insertion of a 3.2-kb DNA fragment or a smaller 0.8-kb DNA fragment derived from...

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Identifiers

PMID: 1429472
DOI: 10.1128/jb.174.22.7486-7489.1992
PMCID: PMC207450

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A region of the broad-host-range plasmid RK2 causes stable in planta inheritance of plasmids in Rhizobium meliloti cells isolated from alfalfa root nodules.PMID 1429472Read ↗

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Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers.Semantic proximity Read ↗

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The host range of RK2 minimal replicon copy-up mutants is limited by species-specific differences in the maximum tolerable copy number.Semantic proximity Read ↗

Identification of a potential membrane-targeting region of the replication initiator protein (TrfA) of broad-host-range plasmid RK2.Semantic proximity Read ↗

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