Article
Irregular polymerase chain reaction-sequence-specific oligonucleotide hybridization patterns reveal seven new HLA-DRB1 alleles related to DR2, DR3, DR6, DR8, and DR11. Implications for sequence-specific priming.
Human immunology - 1 Jan 1995
Anholts J D, Verduyn W, Parlevliet A, Doxiadis I I, D'Amaro J, Giphart M J, Persijn G G, Schreuder G M
Abstract excerpt
In the past 3 years we have typed over 7000 individuals for HLA-DRB using a nonradioactive PCR-SSO method. The use of locally developed computer programs simplified data input and the interpretation of the DRB PCR-SSO readings. In this way we detected a number of samples with unexpected hybridiza...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Biotin
- Gene Conversion
- Genetic Variation
- HLA-DQ Antigens
- HLA-DQ beta-Chains
- HLA-DR Antigens
- HLA-DR Serological Subtypes
- HLA-DR2 Antigen
- HLA-DR3 Antigen
- HLA-DR6 Antigen
- HLA-DRB1 Chains