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Article

HLA-DRB1*01 subtyping by allele-specific PCR amplification: a sensitive, specific and rapid technique.

Tissue antigens - 1 May 1991

Olerup O, Zetterquist H

Abstract excerpt

The two DR1-associated cellular specificities Dw1 and Dw20, as well as DR'Br' (Dw'BON'), cannot be unequivocally assigned by serological typing or restriction fragment length polymorphism (RFLP) analysis. We have developed and compared two polymerase chain reaction-based (PCR) typing methods for distinguishing these DRB1 alleles; allele-specific amplification of DRB1*01 alleles followed by an agarose gel...

Topics

Alleles
Base Sequence
DNA
Electrophoresis, Agar Gel
Gene Amplification
HLA-DR Antigens
HLA-DRB1 Chains
Heterozygote
Histocompatibility Antigens Class II
Humans
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Oligonucleotides

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Source

Identifiers

PMID: 1685264
DOI: 10.1111/j.1399-0039.1991.tb01872.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1685264-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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HLA-DRB1*01 subtyping by allele-specific PCR amplification: a sensitive, specific and rapid technique.PMID 1685264Read ↗

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