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Article

PCR-SSO typing for HLA-DRB alleles.

European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Jan 2000

Vaughan R W

Abstract excerpt

Polymerase chain reaction (PCR) amplification of the second exon of DRB genes from genomic DNA is described. Sequence-specific oligonucleotide (SSO) probes have been designed to allow for the typing of DRB alleles. The methods are described in detail and the results of a trial on both homozygous typing cells and restriction fragment length polymorphism (RFLP)-typed local caucasoid controls are given. The ease of...

Topics

Alleles
Base Sequence
DNA Probes
Exons
HLA-DR Antigens
Histocompatibility Testing
Humans
Molecular Sequence Data
Oligodeoxyribonucleotides
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1676912
DOI: 10.1111/j.1744-313x.1991.tb00007.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1676912-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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