Article
Direct sequencing of SSP-PCR-amplified cDNA to identify new alleles in the DR52-associated DRB1 group: identification of DRB1*1115, DRB1*1117 and DRB1*1319.
Tissue antigens - 1 May 1995
Robbins F, Tang T, Yao H, Ng J, Hartzman R J, Hurley C K
Abstract excerpt
Low and high resolution sequence specific oligonucleotide probe hybridization patterns were used to design an approach to direct sequencing of allele specific amplified cDNA. Several PCR amplifications were used to derive overlapping sequence fragments to define complete first domain sequences fo...
Topics
- Alleles
- Base Sequence
- Cell Line, Transformed
- DNA, Complementary
- Genetic Variation
- HLA-DR Antigens
- HLA-DRB1 Chains
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sequence Homology, Nucleic Acid