Article
Detection of DNA mutations in acid formalin-fixed paraffin-embedded archival tumor specimens by polymerase chain reaction-single strand conformation polymorphism analysis.
Cancer detection and prevention - 1 Jan 1995
Marchetti A, Merlo G, Buttitta F, Pellegrini S, Callahan R, Bistocchi M, Squartini F
Abstract excerpt
A single strand conformation polymorphism analysis on polymerase chain reaction (PCR)-amplified genomic DNA was used for the detection of DNA mutations in acid formalin-fixed, paraffin-embedded tissues. Fifty non-small cell lung cancers were screened for mutations in the exon 5 of the p53 tumor s...
Topics
- Base Sequence
- Carcinoma, Non-Small-Cell Lung
- DNA
- Fixatives
- Formaldehyde
- Genes, p53
- Humans
- Lung Neoplasms
- Molecular Sequence Data
- Mutation
- Paraffin Embedding
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Retrospective Studies
- Tissue Fixation