Article
Detection of DNA aberrations in human cancers by single-strand conformation polymorphism analysis of polymerase chain reaction products.
The Tohoku journal of experimental medicine - 1 Oct 1992
Murakami Y, Suzuki Y, Kishimoto Y, Hirohashi S, Hayashi K, Sekiya T
Abstract excerpt
We have developed a simple, sensitive method, single-strand conformation polymorphism (SSCP) analysis, to detect a single nucleotide substitution in a DNA fragment amplified and labeled by the polymerase chain reaction (PCR). Mobility shift of single-stranded DNAs due to their specific conformati...
Topics
- Carcinoma, Hepatocellular
- Chromosome Aberrations
- DNA, Neoplasm
- DNA, Single-Stranded
- Genes, p53
- Genes, ras
- Humans
- Liver Neoplasms
- Lung Neoplasms
- Mutation
- Nucleic Acid Conformation
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Retinoblastoma