Article
Direct cycle sequencing of mutated alleles detected by PCR single-strand conformation polymorphism analysis.
BioTechniques - 1 May 1993
Mok S C, Lo K W, Tsao S W
Abstract excerpt
A rapid protocol for direct sequencing of a mutated allele, detected by combining polymerase chain reaction with single-strand conformation polymorphism (PCR-SSCP) analysis and cycle sequencing using a thermal cycler, is described. End-labeled radioactive primers were used both for PCR-SSCP analy...
Topics
- Alleles
- Base Sequence
- Biotechnology
- DNA, Neoplasm
- DNA, Single-Stranded
- Female
- Genes, p53
- Humans
- Nucleic Acid Conformation
- Ovarian Neoplasms
- Point Mutation
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Sequence Analysis, DNA