Article
Direct cycle sequencing of mutated alleles detected by PCR single-strand conformation polymorphism analysis.
BioTechniques - 1 May 1993
Mok S C, Lo K W, Tsao S W
Abstract excerpt
A rapid protocol for direct sequencing of a mutated allele, detected by combining polymerase chain reaction with single-strand conformation polymorphism (PCR-SSCP) analysis and cycle sequencing using a thermal cycler, is described. End-labeled radioactive primers were used both for PCR-SSCP analysis for the detection of p53 gene mutation and for cycle sequencing using delta Taq Version 2.0 DNA Polymerase. The...
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