Article
Improved detection of p53 mutations in soft tissue tumors using new gel composition for automated nonradioactive analysis of single-strand conformation polymorphism.
Electrophoresis - 1 Dec 1997
Schneider-Stock R, Haeckel C, Radig K, Pein C D, Roessner A
Abstract excerpt
We report a new nonradioactive method to detect sequence changes, including single-base substitutions through shifts in electrophoretic mobility using an automated fluorescence sequencer (ALFexpress, Pharmacia, Biotech) connected to external cooling equipment. Single strands were identified by in...
Topics
- Autoanalysis
- Exons
- Fluorescence
- Gels
- Genes, p53
- Genetic Testing
- Humans
- Mutation
- Polymorphism, Single-Stranded Conformational
- Reproducibility of Results
- Soft Tissue Neoplasms