Article
[DNA typing for HLA-DR in donor-recipients of cadaveric transplantation].
Zhonghua yi xue za zhi - 1 May 1996
Tan J, Xie T, Xu Q
Abstract excerpt
OBJECTIVE: To accurately allocate donor-recipients of HLA-matching and improve long-term graft survival, genotyping method for HLA-DR alleles by polymerase chain reaction with sequence-specific primers (PCR-SSP) was established and applied to renal transplantation. METHODS: Thirty primers were designed and synthesized according to the HLA-DR nucleotide sequences. Genomic DNAs were prepared by a rapid salting-out...
Topics
- Alleles
- DNA
- Genotype
- Graft Survival
- HLA-DR Antigens
- Histocompatibility Testing
- Humans
- Kidney Transplantation
- Polymerase Chain Reaction
- Retrospective Studies
- Tissue Donors
