Article
Allele shuffling: conjugational transfer, plasmid shuffling and suppressor analysis in Saccharomyces cerevisiae.
Gene - 21 Mar 1995
Sikorski R S, Michaud W A, Tugendreich S, Hieter P
Abstract excerpt
Trans-acting suppressor analysis represents a powerful genetic technique capable of revealing interactions among biochemical pathways in vivo. Suppressor characterization in Saccharomyces cerevisiae has traditionally utilized meiotic segregation for the requisite manipulation of strain genotypes....
Topics
- Alleles
- Anaphase-Promoting Complex-Cyclosome
- Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome
- Cell Cycle Proteins
- Conjugation, Genetic
- Escherichia coli
- Fungal Proteins
- Genetic Techniques
- Genetic Vectors
- Phenotype
- Plasmids
