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Article

Chromosome-scale genetic mapping using a set of 16 conditionally stable Saccharomyces cerevisiae chromosomes.

Genetics - 1 Dec 2008

Reid Robert J D, Sunjevaric Ivana, Voth Warren P, Ciccone Samantha, Du Wendy, Olsen Aileen E, Stillman David J, Rothstein Rodney

Abstract excerpt

We have created a resource to rapidly map genetic traits to specific chromosomes in yeast. This mapping is done using a set of 16 yeast strains each containing a different chromosome with a conditionally functional centromere. Conditional centromere function is achieved by integration of a GAL1 promoter in cis to centromere sequences. We show that the 16 yeast chromosomes can be individually lost in diploid...

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Identifiers

PMID: 18832360
DOI: 10.1534/genetics.108.087999
PMCID: PMC2600922

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