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Article

In vivo site-directed recombination (SDR): An efficient tool to reveal beneficial epistasis.

Methods in enzymology - 1 Jan 2020

Viña-Gonzalez Javier, Alcalde Miguel

Abstract excerpt

Employing the homologous DNA recombination apparatus of Saccharomyces cerevisiae as a dynamic engineering tool allows mutant libraries to be constructed in a rapid and efficient manner. Among the plethora of methods based on the yeast's splicing apparatus, site-directed recombination (SDR) is often useful to gather information from mutations discovered in directed evolution experiments. When using SDR, the target...

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PMID: 32896276
DOI: 10.1016/bs.mie.2020.04.021

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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