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Article

Resolution of multiphasic reactions by the combination of fluorescence total-intensity and anisotropy stopped-flow kinetic experiments.

Biophysical journal - 1 Dec 1994

Otto M R, Lillo M P, Beechem J M

Abstract excerpt

Multiphasic kinetics are often observed in stopped-flow investigations. To characterize further these kinetic phases, we have developed a methodology whereby fluorescence total intensity and anisotropy stopped-flow data can be combined in a single analysis. Fluorescence total intensity and anisotropy are highly interrelated and contain two very complementary forms of information. Total-intensity changes are...

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Identifiers

PMID: 7696490
DOI: 10.1016/S0006-3495(94)80741-6
PMCID: PMC1225637

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