Article
Resolution of multiphasic reactions by the combination of fluorescence total-intensity and anisotropy stopped-flow kinetic experiments.
Biophysical journal - 1 Dec 1994
Otto M R, Lillo M P, Beechem J M
Abstract excerpt
Multiphasic kinetics are often observed in stopped-flow investigations. To characterize further these kinetic phases, we have developed a methodology whereby fluorescence total intensity and anisotropy stopped-flow data can be combined in a single analysis. Fluorescence total intensity and anisot...
Topics
- Bacteriophage T4
- Biophysical Phenomena
- Biophysics
- DNA-Directed DNA Polymerase
- Fluorescence Polarization
- Kinetics
- Micrococcal Nuclease
- Mutation
- Phosphoglycerate Kinase
- Protein Folding
- Saccharomyces cerevisiae
- Spectrometry, Fluorescence