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Article

Enzyme kinetics above denaturation temperature: a temperature-jump/stopped-flow apparatus.

Biophysical journal - 15 Dec 2006

Kintses Bálint, Simon Zoltán, Gyimesi Máté, Tóth Júlia, Jelinek Balázs, Niedetzky Csaba, Kovács Mihály, Málnási-Csizmadia András

Abstract excerpt

We constructed a "temperature-jump/stopped-flow" apparatus that allows us to study fast enzyme reactions at extremely high temperatures. This apparatus is a redesigned stopped-flow which is capable of mixing the reactants on a submillisecond timescale concomitant with a temperature-jump even as large as 60 degrees C. We show that enzyme reactions that are faster than the denaturation process can be investigated...

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Identifiers

PMID: 17012324
DOI: 10.1529/biophysj.106.092833
PMCID: PMC1779911

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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