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Article

Population of on-pathway intermediates in the folding of ubiquitin.

Journal of molecular biology - 28 Jul 2006

Crespo Maria D, Simpson Emma R, Searle Mark S

Abstract excerpt

The role that intermediate states play in protein folding is the subject of intense investigation and in the case of ubiquitin has been controversial. We present fluorescence-detected kinetic data derived from single and double mixing stopped-flow experiments to show that the F45W mutant of ubiquitin (WT*), a well-studied single-domain protein and most recently regarded as a simple two-state system, folds via...

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Identifiers

PMID: 16815444
DOI: 10.1016/j.jmb.2006.05.061

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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