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Article

Pre-Steady-State Kinetic Analysis of Single-Nucleotide Incorporation by DNA Polymerases.

Current protocols in nucleic acid chemistry - 1 Jun 2016

Su Yan, Peter Guengerich F

Abstract excerpt

Pre-steady-state kinetic analysis is a powerful and widely used method to obtain multiple kinetic parameters. This protocol provides a step-by-step procedure for pre-steady-state kinetic analysis of single-nucleotide incorporation by a DNA polymerase. It describes the experimental details of DNA substrate annealing, reaction mixture preparation, handling of the RQF-3 rapid quench-flow instrument, denaturing...

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Identifiers

PMID: 27248785
DOI: 10.1002/cpnc.2
PMCID: PMC4903026

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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