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Article

Continuous assays for DNA translocation using fluorescent triplex dissociation: application to type I restriction endonucleases.

Journal of molecular biology - 13 May 2005

McClelland Sarah E, Dryden David T F, Szczelkun Mark D

Abstract excerpt

Fluorescent assays and accompanying kinetic models are described for the analysis of DNA translocation independent of duplex unwinding. A triplex binding site (TBS) was introduced into DNA substrates at precise loci downstream of recognition sequences for type IA, IB and IC restriction endonucleases (EcoKI, EcoAI and EcoR124I, respectively). Each endonuclease was incubated (without ATP) with substrates on which a...

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PMID: 15843021
DOI: 10.1016/j.jmb.2005.03.018

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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