Article
DNA-sequence specificity of mutations at the human thymidine kinase locus.
Mutation research - 1 Oct 1993
Grosovsky A J, Walter B N, Giver C R
Abstract excerpt
We have established a system for the study of DNA-sequence specificity at a functionally heterozygous thymidine kinase (tk) locus in a human lymphoblastoid cell line (TK6). Characterization of the parental locus demonstrated that the 2 tk alleles were fortuitously distinguished by differential ge...
Topics
- Amino Acid Sequence
- B-Lymphocytes
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Cell Line
- Chromosomes, Human, Pair 17
- DNA Mutational Analysis
- Frameshift Mutation
- Gene Deletion
- Heterozygote
- Humans
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Point Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length