Article
Sequence analysis of tka(-)-1 and tkb(+)-1 alleles in L5178Y tk+/- mouse-lymphoma cells and spontaneous tk-/- mutants.
Mutation research - 1 Apr 1993
Liechty M C, Rauchfuss H S, Lugo M H, Hozier J C
Abstract excerpt
The mouse-lymphoma mutagenesis assay detects forward mutations at the heterozygous thymidine kinase (tk-1) locus in L5178Y tk+/- 3.7.2C cells. This assay of genotoxicity is widely used to quantitate the mutagenic potential of a variety of chemical and physical agents. A NcoI heteromorphism betwee...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Southern
- DNA Mutational Analysis
- DNA, Neoplasm
- Exons
- Heterozygote
- Lymphoma
- Mice
- Molecular Sequence Data
- Mutagenicity Tests
- Mutation
- Thymidine Kinase
- Tumor Cells, Cultured