Article
Spectrum of spontaneous HPRT- mutations in TK6 human lymphoblasts.
Environmental and molecular mutagenesis - 1 Jan 1993
Giver C R, Nelson S L, Grosovsky A J
Abstract excerpt
The occurrence of deletions, coding sequence alterations, and intronic changes leading to aberrant splicing has been characterized among 33 spontaneous HPRT- mutants in TK6 human lymphoblasts. Deletions detectable by multiplex PCR amplification accounted for 45% (15/33) of the mutant collection....
Topics
- B-Lymphocytes
- Base Sequence
- Cell Line
- DNA Mutational Analysis
- Frameshift Mutation
- Gene Deletion
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Nucleic Acid Heteroduplexes
- Point Mutation
- Polymerase Chain Reaction
- Sequence Deletion
- Suppression, Genetic