Article
Single amino acid substitution defines a naturally occurring genetic variant of human thymidylate synthase.
Molecular pharmacology - 1 Apr 1990
Barbour K W, Berger S H, Berger F G
Abstract excerpt
Previously, we identified an altered structural form of thymidylate synthase (TS) in a human colonic tumor cell line. This form, which is encoded by a variant structural gene, renders cells relatively resistant to 5-fluoro-2'-deoxyuridine as a result of the reduced affinity of the enzyme for the...
Topics
- Amino Acid Sequence
- Blotting, Northern
- DNA
- DNA Mutational Analysis
- DNA, Neoplasm
- Histidine
- Humans
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Thymidylate Synthase
- Tumor Cells, Cultured
- Tyrosine