Article
A system for assaying homologous recombination at the endogenous human thymidine kinase gene.
Proceedings of the National Academy of Sciences of the United States of America - 1 Aug 1991
Benjamin M B, Potter H, Yandell D W, Little J B
Abstract excerpt
A system for assaying human interchromosomal recombination in vitro was developed, using a cell line containing two different mutant thymidine kinase genes (TK) on chromosomes 17. Heteroalleles were generated in the TK+/+ parent B-lymphoblast cell line WIL-2 by repeated exposure to the alkylating...
Topics
- Amino Acid Sequence
- Base Sequence
- Cell Line
- Exons
- Genes
- Humans
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Restriction Fragment Length
- Recombination, Genetic
- Thymidine Kinase