Article
Suppression of anchorage-independent growth after gene transfection.
British journal of cancer - 1 Aug 1993
Winterbourne D J, Thomas S, Hermon-Taylor J
Abstract excerpt
A novel procedure for isolating anchorage-dependent cells has been developed. It involves negative selection of cells growing in suspension followed by clonal replica screening for anchorage-dependent growth. Cells which have regained anchorage-dependent growth have been isolated from a library of the Chinese hamster ovary cell line, CHO-K1, transfected with pSV2neo and human genomic DNA. One anchorage-dependent...
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