Article
Antisense RNA-mediated deficiency of the calpain protease, nCL-4, in NIH3T3 cells is associated with neoplastic transformation and tumorigenesis.
The Journal of biological chemistry - 6 Oct 2000
Liu K, Li L, Cohen S N
Abstract excerpt
We previously have described the use of an antisense RNA strategy termed random homozygous knock-out (RHKO) to identify negative regulators of cell proliferation. Here we report the discovery that RHKO-mediated deficiency of the nCL-4 calpain protease results in cellular transformation of and tumorigenesis by murine NIH3T3 fibroblasts. We isolated cell clones able to form colonies on 0.5% soft agar and found that...
Topics
- 3T3 Cells
- Alleles
- Animals
- Base Sequence
- Blotting, Southern
- Blotting, Western
- Calpain
- Cell Division
- Cell Transformation, Neoplastic
- Cytomegalovirus
- DNA, Complementary
- Female
- Gene Library
- Mice