Article
The conserved terminal guanosine of a group I intron can help prevent reopening of the ligated exons.
Journal of molecular biology - 20 Jul 1993
Suh E, Waring R B
Abstract excerpt
The highly conserved terminal guanosine of the Tetrahymena group I intron was mutated to an adenosine. The intron still excised itself but more slowly than the wild-type. At very low concentrations of GTP only ligated exons were produced, but at high concentrations of GTP unligated exons accumulated and the 3' exon acquired a GTP at its 5' end. A series of experiments suggested that GTP was primarily reopening...
Topics
- Animals
- Base Sequence
- Conserved Sequence
- Exons
- Guanosine
- Guanosine Triphosphate
- Introns
- Molecular Sequence Data
- Mutation
- RNA
- RNA Splicing
- Ribonucleases