Article
The high mutator activity of the dnaQ49 allele of Escherichia coli is medium-dependent and results from both defective 3'-->5' proofreading and methyl-directed mismatch repair.
Mutation research - 1 Aug 1993
Krishnaswamy S, Rogers J A, Isbell R J, Fowler R G
Abstract excerpt
The Escherichia coli dnaQ49 mutator allele maps at the dnaQ locus, the structural gene for the epsilon subunit of the DNA polymerase III holoenzyme. Epsilon, when bound to the alpha subunit, provides the 3'-->5' exonuclease activity (proofreading) that removes 3' mismatched terminal nucleotides from the nascent DNA strand during replication. The temperature sensitive dnaQ49 allele lacks this catalytic activity...
Topics
- Alleles
- Culture Media
- DNA Polymerase III
- DNA Repair
- Escherichia coli
- Genes, Bacterial
- Mutation