Article
A strong mutator effect caused by an amino acid change in the alpha subunit of DNA polymerase III of Escherichia coli.
The Journal of biological chemistry - 15 Mar 1991
Maki H, Mo J Y, Sekiguchi M
Abstract excerpt
Most potent mutators heretofore detected in Escherichia coli are associated with defects in epsilon subunit of DNA polymerase III, encoded by the dnaQ gene. To elucidate the role of the alpha subunit, the catalytic subunit of the polymerase, in maintaining the high fidelity of DNA replication, we isolated a mutator mutant, the mutation (dnaE173) of which resides on the dnaE gene, encoding the alpha subunit. The...
Topics
- Alleles
- Amino Acids
- Chimera
- Chromatography, Gel
- DNA Polymerase III
- DNA Replication
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genes, Bacterial
- Genetic Linkage