Article
Effect of O6-alkylguanine-DNA alkyltransferase on the frequency and spectrum of mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in the HPRT gene of diploid human fibroblasts.
Mutation research - 1 Jan 2000
Lukash L L, Boldt J, Pegg A E, Dolan M E, Maher V M, McCormick J J
Abstract excerpt
N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) reacts with 12 nucleophilic sites in DNA to induce a variety of lesions, but O6-methylguanine (O6-MeG) and O4-methylthymine are the most effective premutagenic lesions produced, mispairing with thymine and guanine, respectively. O6-MeG is repaired by O6...
Topics
- Base Sequence
- Cell Line
- DNA
- DNA Mutational Analysis
- Diploidy
- Drug Interactions
- Fibroblasts
- Guanine
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Infant, Newborn
- Methylnitronitrosoguanidine
- Methyltransferases
- Molecular Sequence Data
- Mutation