Article
A dominant negative Raf-1 mutant prevents v-Src-induced transformation.
Biochemical and biophysical research communications - 30 Apr 1993
Qureshi S A, Joseph C K, Hendrickson M, Song J, Gupta R, Bruder J, Rapp U, Foster D A
Abstract excerpt
A vector expressing a dominant negative mutant of Raf-1 was stably introduced into BALB/c 3T3 cells expressing a temperature-sensitive derivative of v-Src. High levels of the Raf-mutant were detected in these cells. Expression of the Raf-1 mutant blocked v-Src-induced transformation, as determine...
Topics
- 3T3 Cells
- Animals
- Cell Transformation, Neoplastic
- Cells, Cultured
- Diglycerides
- Genes, Dominant
- Mice
- Mice, Inbred BALB C
- Mutation
- Oncogene Protein pp60(v-src)
- Phosphotyrosine
- Protein Serine-Threonine Kinases
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-raf
- Tyrosine