Article
Regulation of Raf-1 and Raf-1 mutants by Ras-dependent and Ras-independent mechanisms in vitro.
Molecular and cellular biology - 1 Aug 1995
Dent P, Reardon D B, Morrison D K, Sturgill T W
Abstract excerpt
The serine/threonine kinase Raf-1 functions downstream from Ras to activate mitogen-activated protein kinase kinase, but the mechanisms of Raf-1 activation are incompletely understood. To dissect these mechanisms, wild-type and mutant Raf-1 proteins were studied in an in vitro system with purifie...
Topics
- 3T3 Cells
- Adenosine Triphosphate
- Animals
- Cell Membrane
- Cell Transformation, Neoplastic
- Enzyme Activation
- Guanosine Triphosphate
- Mice
- Mutation
- Oligopeptides
- Peptides
- Phosphorylation
- Protein Serine-Threonine Kinases
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-raf
- Recombinant Fusion Proteins
- Signal Transduction
- ras Proteins