Conformational changes in mutant lysozymes detected with monoclonal antibody.

doi:10.1016/0014-5793(95)00846-2

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Article

Conformational changes in mutant lysozymes detected with monoclonal antibody.

FEBS letters - 28 Aug 1995

Kato A, Shimizu T, Saga S

Abstract excerpt

A monoclonal antibody (mAb) against hen egg white lysozyme (HEWL) with the exquisitely sensitive specificity to native conformation was prepared to detect the conformational changes in mutant lysozymes constructed by genetic modification in a yeast expression system. The binding of mAb with lysoz...

Topics

Animals
Antibodies, Monoclonal
Antibody Specificity
Binding, Competitive
Guanidine
Guanidines
Hot Temperature
Male
Mice
Mice, Inbred BALB C
Muramidase
Mutation
Protein Conformation
Protein Denaturation
Protein Folding
Saccharomyces cerevisiae

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