Article
Properties of monoclonal antibodies selected for probing the conformation of wild type and mutant forms of the P22 tailspike endorhamnosidase.
The Journal of biological chemistry - 25 Jun 1990
Friguet B, Djavadi-Ohaniance L, Haase-Pettingell C A, King J, Goldberg M E
Abstract excerpt
Eleven species of monoclonal antibodies directed against the trimeric P22 tailspike endorhamnosidase have been selected and characterized. Seven of these antibodies recognize the native tailspike, both isolated and assembled onto the virion, and prevent phage infection. Four antibodies react with...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Centrifugation, Density Gradient
- Enzyme-Linked Immunosorbent Assay
- Glycoside Hydrolases
- Mutation
- Protein Binding
- Protein Conformation
- Protein Denaturation
- Salmonella Phages
- Salmonella typhimurium
- Viral Proteins
- Viral Tail Proteins