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Article

Thermostability of doubly glycosylated recombinant lysozyme.

Biological & pharmaceutical bulletin - 1 Oct 2001

Hashimoto Y, Munemura O, Masumoto K, Ueda T, Imoto T

Abstract excerpt

We prepared a lysozyme mutant (Q41S/R61S) introducing Asn-type glycosylation signal sites by yeast expression system. On purification by cation exchange column at pH 7, three fractions were obtained. Peptide mapping and mass-spectrometry showed the fractions were the derivatives glycosylated at both Asn39 and Asn59, at only Asn39, and not glycosylated. It was revealed that the processing of Asn-linked...

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Source

Identifiers

PMID: 11642311
DOI: 10.1248/bpb.24.1102

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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