Article
Stabilization of lysozyme by introducing N-glycosylation signal sequence.
Journal of biochemistry - 1 Jan 1996
Ueda T, Iwashita H, Hashimoto Y, Imoto T
Abstract excerpt
We designed mutant lysozymes with N-glycosylation signal sequences (Asn48-Gly49-Thr-50 and Asn87-Ile88-Thr89) by substituting Asp to Asn at positions 48 and 87. When these mutant lysozymes were expressed by using yeast (Saccharomyces cerevisiae) in Burkholder minimum medium, N-glycosylation occur...
Topics
- Amino Acid Sequence
- Asparagine
- Aspartic Acid
- Base Sequence
- Enzyme Stability
- Glycosylation
- Guanidine
- Guanidines
- Molecular Sequence Data
- Muramidase
- Mutation
- Protein Conformation
- Protein Denaturation
- Protein Folding
- Protein Sorting Signals
- Recombinant Proteins
- Saccharomyces cerevisiae
- Structure-Activity Relationship