Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Structural and functional properties of hen egg-white lysozyme deamidated by protein engineering.

Bioscience, biotechnology, and biochemistry - 1 Sept 1992

Kato A, Tanimoto S, Muraki Y, Kobayashi K, Kumagai I

Abstract excerpt

The structural and functional properties of lysozymes genetically deamidated at positions 103 (N103D) and 106 (N106D) were studied by a protein engineering technique. The wild-type and mutant lysozymes were expressed in Saccharomyces cerevisiae and purified from the cultivation medium in two steps by cation-exchange chromatography on CM-Toyopearl. The lytic activity of deamidated lysozymes was almost the same as...

Topics

Animals
Base Sequence
Chickens
Circular Dichroism
Deamination
Egg Proteins
Hydrogen-Ion Concentration
Molecular Sequence Data
Muramidase
Mutation
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Conformation

Protein Engineering

Saccharomyces cerevisiae

Structure-Activity Relationship

Transfection

Source

Identifiers

PMID: 1368949
DOI: 10.1271/bbb.56.1424

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1368949-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Structural and functional properties of hen egg-white lysozyme deamidated by protein engineering.PMID 1368949Read ↗

Stabilization of lysozyme by introducing N-glycosylation signal sequence.Semantic proximity Read ↗

Effect of N-terminal mutation of human lysozyme on enzymatic activity.Semantic proximity Read ↗

Role of His101 in the Protein Folding/Unfolding of a Goose-Type Lysozyme from Ostrich (Struthio camelus) Egg White.Semantic proximity Read ↗

Thermostability of doubly glycosylated recombinant lysozyme.Semantic proximity Read ↗

Design and structural analysis of an engineered thermostable chicken lysozyme.Semantic proximity Read ↗

Effect of chicken lysozyme signal peptide alterations on secretion of human lysozyme in Saccharomyces cerevisiae.Semantic proximity Read ↗

The mutational effect of Ile58 at subsite C in hen egg-white lysozyme on substrate binding, enzymatic activity, and protein stability.Semantic proximity Read ↗

Effect of foreign N-terminal residues on the conformational stability of human lysozyme.Semantic proximity Read ↗

Increasing the hydrophobic interaction between terminal W-motifs enhances the stability of Salmonella typhimurium sialidase. A general strategy for the stabilization of beta-propeller protein fold.Semantic proximity Read ↗

Amino acid sequence of Egyptian goose egg-white lysozyme and effects of amino acid substitution on the enzymatic activity.Semantic proximity Read ↗

[Functional analysis of lysozyme by conversion of amino acid residues].Semantic proximity Read ↗

The crystal structure of a mutant human lysozyme C77/95A with increased secretion efficiency in yeast.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.