Article
Production of genetically modified lysozymes having extreme heat stability and antimicrobial activity against gram negative bacteria in yeast and in plant.
Die Nahrung - 1 Aug 1998
Kato A, Nakamura S, Ibrahim H, Matsumi T, Tsumiyama C, Kato M
Abstract excerpt
Hen egg white lysozyme was genetically modified to have extreme heat stability and strong antimicrobial activity against Gram negative bacteria and the modified lysozymes were secreted in yeast and tobacco. Complementary DNA encoding lysozyme was subjected to site-directed mutagenesis to have the...
Topics
- Anti-Bacterial Agents
- DNA
- Genetic Vectors
- Gram-Negative Bacteria
- Muramidase
- Mutation
- Plants, Toxic
- Polymerase Chain Reaction
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Nicotiana
- Transformation, Genetic