Article
Characterization and epitope mapping of four HLA class II reactive mouse monoclonal antibodies using transfected L cells and human cells transfected with mutants of DQB1*0302.
Tissue antigens - 1 Apr 1995
Viken H D, Thorsby E, Gaudernack G
Abstract excerpt
To study epitopes of HLA class II molecules, four mouse monoclonal antibodies (mAbs) 13B6, 17F8, 19A1 and 12G6 were made using HLA-DQ8, DP2 and DP4 expressing mouse transfectants for immunization. Three of the mAbs, 13B6, 17F8 and 19A1, bound to all DQ1, 4, 8 or 9 positive B-lymphoblastoid cell lines (B-LCLs) and transfectants tested, i.e. cells carrying the DQB1 genes 0302-3, 0401-2, 0501-3, 0601-4 and 0609...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Epitope Mapping
- HLA-D Antigens
- HLA-DQ Antigens
- Histocompatibility Antigens Class II
- Humans
- L Cells
- Mice
- Mice, Inbred BALB C