Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Production of epitope specific monoclonal IgG antibodies to HLA class II molecules by combining in vivo and in vitro immunization.

Journal of immunological methods - 6 Jul 1992

Petersen J S, Dyrberg T

Abstract excerpt

To study the expression of HLA-DQ beta chain alleles associated with type 1 diabetes, mAbs were generated from mice immunized with synthetic peptides representing allelic HLA-DQw7 and HLA-DQw8 beta chain sequences. The splenocytes from immunized mice were fused with myeloma cells, either immediately after or following additional in vitro boosting with peptide. Peptide-specific mAbs, predominantly of the IgG...

Topics

Alleles
Amino Acid Sequence
Antibodies, Monoclonal
Antibody Specificity
Blotting, Western
Epitopes
Flow Cytometry
HLA-D Antigens
Humans
Immunoglobulin G
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Peptides

Source

Identifiers

PMID: 1378472
DOI: 10.1016/0022-1759(92)90102-y

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1378472-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Production of epitope specific monoclonal IgG antibodies to HLA class II molecules by combining in vivo and in vitro immunization.PMID 1378472Read ↗

A rapid and efficient strategy to generate allele-specific anti-HLA monoclonal antibodies.Semantic proximity Read ↗

Mapping the epitopes of antibodies.Semantic proximity Read ↗

Identification of the amino acid residues contributing to monoclonal antibody-defined DQw1 epitopes.Semantic proximity Read ↗

Characterization of a murine monoclonal antibody recognizing HLA-DQ5(1), DQ6(1) and DQ4 antigens.Semantic proximity Read ↗

Generation and reactivity analysis of human recombinant monoclonal antibodies directed against epitopes on HLA-DRSemantic proximity Read ↗

Use of monoclonal antibodies to detect disease associated HLA-DRB1 alleles and the shared epitope in rheumatoid arthritis.Semantic proximity Read ↗

Characterization and epitope mapping of four HLA class II reactive mouse monoclonal antibodies using transfected L cells and human cells transfected with mutants of DQB1*0302.Semantic proximity Read ↗

MHC class II isotype- and allele-specific attenuation of experimental autoimmune encephalomyelitis.Semantic proximity Read ↗

Alloreactive monoclonal antibodies select Kd molecules with different peptide profiles.Semantic proximity Read ↗

HLA antibody responses in HLA class I transgenic mice.Semantic proximity Read ↗

Epitope mapping of human monoclonal antibodies to HLA-B27 by using natural and mutated antigenic variants.Semantic proximity Read ↗

T cells recognizing an HLA-DQ alpha beta heterodimer encoded in cis by the DR4DQw4 haplotype and in trans by DR4DQw8/DRw8DQw4 heterozygous cells.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.