Article
Production and characterization of murine monoclonal antibodies recognizing HLA-DQ polymorphisms obtained by immunizing mice with transfected L cells.
Human immunology - 1 Jun 1992
Vitale M, Pistillo M P, Tazzari P L, Falco M, Sun P F, Mantero S, Ferrara G B
Abstract excerpt
Two polymorphic anti-HLA-DQB1 mAbs, TM 902 and TM 903, have been produced by immunizing F1 mice (Balb/C x C3H) with HLA-DQ-transfected mouse L cells. Cytotoxic analysis on a panel of HLA-typed cell lines has shown that TM 902 reacts with all the DQB1* alleles except DQB1*0501, *0502, and *0503, and DQB1*0601, *0602, *0603, and *0604, whereas TM 903 reacts with the DQB1*0501, *0502, and *0503, DQB1*0601, *0602,...
Topics
- Alleles
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Flow Cytometry
- Fluorescent Antibody Technique
- HLA-DQ Antigens
- Immunization
- L Cells
- Mice
- Mice, Inbred BALB C