Production of mouse monoclonal antibodies against human HLA-A3 for use in microlymphocytotoxicity technique.

doi:10.3233/HAB-2009-0201

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Article

Production of mouse monoclonal antibodies against human HLA-A3 for use in microlymphocytotoxicity technique.

Human antibodies - 1 Jan 2009

Yari Fatemeh, Sareh Shahla, Sadri Azadeh, Sharifi Zohreh, Najafi Freshteh

Abstract excerpt

Monoclonal antibody production using the hybridoma technology is one of the attractive areas of research. The aim of this study was to investigate the production of monoclonal antibodies to human HLA-A3 antigen for use in microlymphocytotoxicity technique. HLA-A3 antigen was purified from human leukocytes and injected to several mice. The spleen-derived cells of immunized animal were fused with P3-X63-Ag8 myeloma...

Topics

Alleles
Animals
Antibodies, Monoclonal
Cell Adhesion
Culture Media
Cytotoxicity, Immunologic
Enzyme-Linked Immunosorbent Assay
Female
HLA-A3 Antigen
Humans
Hybridomas
Lymphocytes
Mice
Mice, Inbred BALB C
Spleen

Source

Open in PubMed

Identifiers

PMID: 19729806
DOI: 10.3233/HAB-2009-0201

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