Article
Polymorphic DQ alpha and DQ beta interactions dictate HLA class II determinants of allo-recognition.
The Journal of experimental medicine - 1 Jan 1990
Kwok W W, Mickelson E, Masewicz S, Milner E C, Hansen J, Nepom G T
Abstract excerpt
18 transfected cell lines were generated that expressed distinct DQ molecules related to the serologically defined HLA-DQw3 specificity. These transfectants were constructed using site-directed mutagenesis to introduce nucleotide substitutions into DQ3.2 beta cDNA, followed by retrovirus-mediated...
Topics
- Amino Acid Sequence
- B-Lymphocytes
- Base Sequence
- Cell Line
- Gene Expression
- Genes, MHC Class II
- Genetic Vectors
- HLA-DQ Antigens
- Humans
- Isoantigens
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Polymorphism, Genetic
- Sequence Homology, Nucleic Acid
- T-Lymphocytes
- Transfection