Article
Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.
Journal of bacteriology - 1 Aug 1995
Vonstein V, Johnson S P, Yu H, Casadaban M J, Pagratis N C, Weber J M, Demirjian D C
Abstract excerpt
Mutants of the extreme thermophile Thermus flavus in the pyrimidine biosynthetic pathway (Pyr-) were isolated by resistance to 5-fluoroorotic acid. The pyrE gene, which codes for the orotate phosphoribosyltransferase, was cloned by recombination with one of the isolated Pyr- T. flavus mutant strains. It was subcloned by complementation of an Escherichia coli pyrE mutant strain and was sequenced. The deduced...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- DNA, Bacterial
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Orotate Phosphoribosyltransferase
- Thermus