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Article

Construction of a shuttle vector for, and spheroplast transformation of, the hyperthermophilic archaeon Pyrococcus abyssi.

Applied and environmental microbiology - 1 Nov 2002

Lucas Soizick, Toffin Laurent, Zivanovic Yvan, Charlier Daniel, Moussard Hélène, Forterre Patrick, Prieur Daniel, Erauso Gaël

Abstract excerpt

Our understanding of the genetics of species of the best-studied hyperthermophilic archaea, Pyrococcus spp., is presently limited by the lack of suitable genetic tools, such as a stable cloning vector and the ability to select individual transformants on plates. Here we describe the development of a reliable host-vector system for the hyperthermophilic archaeon Pyrococcus abyssi. Shuttle vectors were constructed...

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Identifiers

PMID: 12406746
DOI: 10.1128/AEM.68.11.5528-5536.2002
PMCID: PMC129897

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