Article
Interallelic complementation at the pyrF locus and the homodimeric nature of orotate phosphoribosyltransferase (OPRTase) in Mucor circinelloides.
Molecular & general genetics : MGG - 1 Nov 1998
Velayos A, Alvarez M I, Eslava A P, Iturriaga E A
Abstract excerpt
Using 5-fluoroorotic acid (5-FOA) as a positive selection system we isolated mutants of Mucor circinelloides altered in the pyrimidine biosynthetic pathway. These mutants were found to be deficient either in orotidine-5'-monophosphate decarboxylase (OMPdecase), or in orotate phosphoribosyltransfe...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Dimerization
- Fungal Proteins
- Genetic Complementation Test
- Models, Molecular
- Molecular Sequence Data
- Mucor
- Mutation
- Orotate Phosphoribosyltransferase
- Orotic Acid
- Protein Conformation
- Pyrimidines
- Sequence Analysis
- Transformation, Genetic