Article
Characterization of intragenic recombination in a hyperthermophilic archaeon via conjugational DNA exchange.
Journal of bacteriology - 1 May 2001
Reilly M S, Grogan D W
Abstract excerpt
Sulfolobus acidocaldarius is so far the only hyperthermophilic archaeon in which genetic recombination can be assayed by conjugation and simple selections. Crosses among spontaneous pyr mutants were able to resolve closely spaced chromosomal mutations, identify deletions and rearrangements, and map mutations to a given deletion interval. Frameshift mutations in pyrE exerted polar effects that depressed...
Topics
- Alleles
- Archaeal Proteins
- Bacterial Proteins
- Conjugation, Genetic
- DNA-Binding Proteins
- Drug Resistance, Microbial
- Frameshift Mutation
- Genes, Archaeal
- Integration Host Factors
- Orotic Acid
- Promoter Regions, Genetic
- Pseudomonas
- Recombination, Genetic
- Sulfolobus acidocaldarius
- Temperature
- Trans-Activators
- Transcription Factors