Article
Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus.
Current genetics - 1 Dec 1993
Campuzano V, del Valle P, de Vicente J I, Eslava A P, Alvarez M I
Abstract excerpt
A total of seven pyrimidine auxotrophs of Phycomyces were isolated from among 5-fluoroorotate acid (5-FOA)-resistant mutants. They were classified by complementation into two groups. A representative mutant strain belonging to one group was deficient in orotate phosphoribosyl transferase (OPRTase...
Topics
- Cell-Free System
- Drug Resistance, Microbial
- Genetic Complementation Test
- Genetic Linkage
- Genetic Markers
- Genotype
- Orotate Phosphoribosyltransferase
- Orotic Acid
- Phycomyces
- Pyrimidines
- Recombination, Genetic
- Restriction Mapping
- Spores, Fungal
