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Disruption of Thermus thermophilus genes by homologous recombination using a thermostable kanamycin-resistant marker.

FEBS letters - 12 Oct 2001

Hashimoto Y, Yano T, Kuramitsu S, Kagamiyama H

Abstract excerpt

Genes of an extremely thermophilic bacterium, Thermus thermophilus, were disrupted by homologous recombination using a recently developed, thermostable kanamycin-resistant marker. First, the trpE gene was disrupted with various constructions of DNA. The transformation efficiency was exponentially increased as the length of the homologous regions flanking the marker gene increased above the minimum length (200-300...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 11602251
DOI: 10.1016/s0014-5793(01)02926-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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