Article
Site-directed mutants of the beta subunit of protein kinase CK2 demonstrate the important role of Pro-58.
FEBS letters - 17 Jul 1995
Hinrichs M V, Gatica M, Allende C C, Allende J E
Abstract excerpt
The following amino acids of the Xenopus laevis beta subunit of protein kinase CK2 (casein kinase 2) were changed to alanine: Pro-58 (beta P-->A); Asp-59 and Glu-60 and Glu-61 (beta DEE-->AAA); His-151-153 (beta HHH-->AAA). The last 37 amino acids of the carboxyl end were deleted (beta delta 179-215). Stimulation of CK2 alpha catalytic subunit activity was measured with casein as substrate and the following...
Topics
- Animals
- Base Sequence
- Casein Kinase II
- Caseins
- DNA
- DNA-Binding Proteins
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Phosphorylation
- Proline
- Protein Conformation