Article
Casein kinase 2 down-regulation and activation by polybasic peptides are mediated by acidic residues in the 55-64 region of the beta-subunit. A study with calmodulin as phosphorylatable substrate.
Biochemistry - 12 Apr 1994
Meggio F, Boldyreff B, Issinger O G, Pińna L A
Abstract excerpt
The noncatalytic beta-subunit is responsible for the latency of casein kinase 2 (CK2) activity toward calmodulin. Twenty-one mutants of the beta-subunit bearing either deletions or Ala substitutions for charged residues in the 5-6, 55-70, and 171-178 sequences have been assayed for their ability...
Topics
- Amino Acid Sequence
- Calmodulin
- Casein Kinases
- Down-Regulation
- Enzyme Activation
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Mutation
- Peptide Fragments
- Phosphorylation
- Polyamines
- Polyelectrolytes
- Polymers
- Protein Kinases